P.083 Liquid biopsies reveal brain cell death in central nervous system tumors

Background: Circulating cell-free DNA (cfDNA) is a novel type of biomarker with broad utility in diagnostic medicine, based on the release fragments from dying cells to circulation. We developed an approach for identifying tissue origins cfDNA, using cell-type-specific methylation patterns, massive reference atlas genome-wide methylomes multiple human tissues and cell types. Cancer inflicts damage surrounding normal tissues, which can culminate fatal organ failure. demonstrated that brain death CNS cancer be detected by tissue-specific patterns circulating cfDNA. Methods: cocktail brain-specific markers, used it assess presence brain-derived-cfDNA plasma patients metastasis. Results: identified significantly elevated neuron-, oligodendrocyte-, astrocyte-derived cfDNA (p<0.0001) metastases (n=29) compared without metastasis (n=113). Conclusions: show new set biomarkers identify high specificity resolution. (neurons, oligodendrocytes, astrocytes) Cell-type-specific markers allow identification collateral damage, reveals metastases, potentially assist early detection.